effects of light-emitting diode irradiation on the osteogenesis of human umbilical cord mesenchymal stem cells in vitro
Effects of light biological regulation therapy on proliferation and division of human umbilical cord-derived stem cells (hUMSCs)
Culture in bone-forming media.
The HUMSCs were irradiated with LED lights of 620nm and 2 j/cm 2, and cell proliferation and bone-forming activity were monitored.
The experiment included four groups of cells: control group;
Bone formation group (osteo group); the LED group;
Bone-forming LED group (
LED group osteo).
Detection of HUMSC proliferation by performing a3-(4,5-Dinazol-2yl)-
2,5 diphenoxazole bromide (MTT)assay.
By alkaline phosphate (ALP)
Stained with Von Kossa, bone Bridge protein (OPN)
Evaluation of gene mRNA expression by reverse transcription PCR (RT-PCR).
HUMSCs in the LED domosteo group showed a much higher proliferation rate than other subgroups.
Besides, there are more people
The positive cells and the von Cosa tuberculosis LED alimtusosteo group.
The expression of bone Bridge protein mRNA in the LED alimosteo group was higher than that in other subgroups.
In conclusion, low-level LED light with a wavelength of 620nm during long culture enhanced the proliferation and bone formation of hUMSCs.